Circulating tumor DNA could represent a minimally invasive approach for detecting AKT pathway alterations in hormone receptor–positive breast cancer.
An analysis of data from the phase 3 CAPItello-291 trial (NCT04305496) that evaluated capivasertib (Truqap) plus fulvestrant (Faslodex) in patients with hormone receptor–positive breast cancer showed that circulating tumor DNA (ctDNA) could represent a minimally invasive option for detecting PIK3CA, AKT1, and PTEN alterations in this patient population when suitable tissue for analysis is unavailable.1
Findings presented at the
When comparing tissue vs ctDNA testing, 207 patients were positive for PIK3CA/AKT1/PTEN alterations in both ctDNA and tissue; 30 were positive in ctDNA and negative in tissue; 42 were positive in ctDNA with unknown tissue status; 63 were positive via tissue and negative with ctDNA; and 17 were positive in tissue with unknown ctDNA status. This translated to an overall percentage agreement (OPA) of 83.3% and a percentage positive agreement (PPA) of 76.7%.
For PIK3CA alterations specifically, the OPA and PPA were 88.9% and 77.1%, respectively. These respective rates were 98.7% and 83.8% when looking at AKT1 alterations. The OPA and PPA were 90.9% and 54.2%, respectively, in regard to PTEN alterations.
“If we look at association of tumor fraction, we can improve the ability to identify patients with low ctDNA shedding, where ctDNA testing alone may miss alterations,” lead study author Nicholas Turner, MD, PhD, FRCP, FMedSci, said in a presentation of the data.
Turner is head of the Ralph Lauren Centre for Breast Cancer Research Breast Unit, director of Clinical Research, and director of The Royal Marsden and Institute of Cancer Research NIHR Biomedical Research Centre in London, United Kingdom.
Findings published in the New England Journal of Medicine showed that in the overall trial population, those treated with capivasertib plus fulvestrant (n = 355) achieved a median progression-free survival (PFS) of 7.2 months (95% CI, 5.5-7.4) compared with 3.6 months (95% CI, 2.8-3.7) for those given placebo plus fulvestrant (n = 353; HR, 0.60; 95% CI, 0.51-0.71; P < .001).2 For patients harboring AKT pathway alterations, the median PFS was 7.3 months (95% CI, 5.5-9.0) with capivasertib plus fulvestrant (n = 155) vs 3.1 months (95% CI, 2.0-3.7) for placebo plus fulvestrant (n = 134; HR, 0.50; 95% CI, 0.38-0.65; P < .001).
Based on these prior data, in November 2023,
The randomized, double-blind, placebo-controlled trial enrolled patients at least 18 years of age with hormone receptor–positive, HER2-negative, locally advanced or metastatic breast cancer who experienced disease progression following prior treatment with an aromatase inhibitor, with or without a CDK4/6 inhibitor.2 Up to 2 previous lines of endocrine therapy and 1 prior line of chemotherapy in the advanced setting were permitted.
Patients were randomly assigned 1:1 to receive capivasertib 400 mg twice per day for 4 days on and 3 days off plus fulvestrant at 500 mg once every 14 days; or placebo plus fulvestrant in 28-day cycles.
Investigator-assessed PFS in the overall population and patients with AKT pathway alterations served as the trial’s primary end point. Secondary end points included overall survival, objective response rate, and safety.
The PFS benefit observed with the capivasertib regimen was consistent across ctDNA/tissue subgroups.1 This included those with PIK3CA/AKT1/PTEN alterations detected by tissue only (HR, 0.50; 95% CI, 0.38-0.65), all patients with evaluable ctDNA (HR, 0.59; 95% CI, 0.50-0.71), those with PIK3CA/AKT1/PTEN alterations detected via ctDNA (HR, 0.43; 95% CI, 0.33-0.56), patients with ESR1 co-mutations detected via ctDNA (HR, 0.52; 95% CI, 0.36-0.76), patients without ESR1 co-mutations (HR, 0.36; 95% CI, 0.23-0.54), patients without PIK3CA/AKT1/PTEN alterations detected in ctDNA (HR, 0.76; 95% CI, 0.60-0.95), patients with PIK3CA/AKT1/PTEN alterations detected in ctDNA or tissue (HR, 0.49; 95% CI, 0.38-0.62), and those without PIK3CA/AKT1/PTEN alterations detected in either tissue or ctDNA (HR, 0.74; 95% CI, 0.58-0.95).
