Ranjit S. Bindra, MD, PhD
Mutations in isocitrate dehydrogenase 1 and 2 (IDH1/2
) are not the mutations commonly found in cancer cells, according to a presentation from the 2017 AACR Annual Meeting.1
These mutations, which are typically found in patients with brain tumors and acute myelogenous leukemia (AML), are not driver mutations that should be targeted with direct IDH inhibitors, but instead they create vulnerabilities that can be exploited through treatment with poly (ADP-ribose) polymerase (PARP) inhibitors. A phase II basket trial has been announced to test this theory in patients with IDH1/2
“Our data strongly suggest that exploiting the DNA repair deficiency in IDH
-mutant tumors, rather than inhibiting the function of the mutant IDH proteins, likely will be a better strategy for treating brain tumors with these specific mutations, a devastating disease with an urgent need for better therapies,” Ranjit S. Bindra, MD, PhD, said in a statement before his presentation.IDH1/2
mutations are heterozygous, missense mutations that induce both a gain- and a loss-of-function phenotype. “The mechanism of action of these mutations is quite fascinating. These are neomorphic mutations that take the normal product of the wild-type enzyme and convert it to this oncometabolite that is foreign to the cell called 2-hydroxyglutarate [2HG],” explained Bindra, assistant professor of therapeutic radiology and experimental pathology, Yale School of Medicine, during the presentation.
Previous research has shown that IDH
mutations may drive the initial formation of tumor cells but quickly become “passenger” mutations once the cells have been transformed.2
As these mutations are not typical driver mutations, they cannot be targeted as such.
Bindra noted that IDH
mutations could serve as either a tumor suppressor gene, like the BRCA1/2
mutations in patients with breast and ovarian cancer—creating a “BRCAness”, or an oncogene, similar to EGFR
mutations. The oncometabolite 2HG, when it comes from mutant IDH1/2
(R-2HG) rather than as a response to hypoxia (S-2HG), suppresses KDM4A/B
and leads to decreased homologous recombination repair and sensitivity to PARP inhibitors, as is often seen in patients with BRCA
mutations. Bindra commented that this could be part of a much larger underlying pathway to genetic instability that could involve other mutations as well.
These findings were previously published by Bindra and his colleagues in Science Translational Medicine
demonstrating the potential sensitivity of IDH
mutations to PARP inhibitors due to the function of the mutation.3
“Our findings raise the question of whether we should target this Achilles heel, meaning should we exploit the DNA repair defect, or should we repair that Achilles heel, that would be in line with the oncometabolite hypothesis. This is a critical question because it really raises questions about how should we be approaching the therapeutic management for these patients,” Bindra said.
The oncometabolite hypothesis suggests that if both growth signals and metabolic rewiring could be blocked, rather than just one or the other, it could have a broad, profound impact on these tumor cells.
Bindra commented that exploitation of the DNA repair defects seen in IDH
mutations is a powerful cytotoxic approach to treating cancer that is potentially a game changer as significant improvements in progression-free survival rates have already been seen with PARP inhibitors, such as olaparib (Lynparza) and talazoparib (BMN-673).
In in vitro studies of IDH1/2
mutations treated with olaparib and talazoparib, a significant difference in survival was found with IDH
mutations versus wild-type tumors favoring the mutant tumor cells. This effect was carried over to in vivo studies with tumor xenograft models where a large difference in survival was noted in treatment with olaparib when an IDH
mutation was lacking.
In addition, Bindra noted that there was a significant synergistic interaction for combination approaches with PARP inhibitors in IDH
-mutant cells. Synergy was marked for a combination of talazoparib and cisplatin as well as for talazoparib and VE-822, an ATR inhibitor.
Alternatively, if IDH1 inhibitors, which are currently under development, were to be used and 2HG would be suppressed, then it would reverse the sensitivity to PARP inhibitors and revert the DNA repair defect.
In preclinical studies of IDH
wild-type and IDH
-mutant cancer cell line models treated with IDH inhibitors or PARP inhibitors, Bindra and his colleagues found that IDH1 inhibitors did not have a significant effect on IDH1
-mutant tumor cell growth whereas PARP inhibitors did.