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Jessica joined the company in August 2019 and is one of the point contacts for the OncLive On Air™ podcast. She is a Rider University alumna and holds a degree in journalism and biology. Prior to joining MJH Life Sciences, she interned with the Ireland-based social media monitoring agency Olytico and served as a copy editor and writer for The Rider News. Email: firstname.lastname@example.org
Pharmacodynamic and pharmacokinetic modeling of the investigational MASP-2 inhibitor narsoplimab supported a weight-based dosing method for patients with hematopoietic stem cell transplant–associated thrombotic microangiopathy.
Pharmacodynamic and pharmacokinetic modeling of the investigational MASP-2 inhibitor narsoplimab (OMS721) supported a weight-based dosing method for patients with hematopoietic stem cell transplant–associated thrombotic microangiopathy (HSCT-TMA), according to findings from an analysis presented during the Virtual 47th Annual Meeting of the EBMT.1
Moreover, the findings demonstrated favorable exposure-response relationships between serum concentrations of narsoplimab and C4d inhibition for HSCT-TMA treatment responder and non-responder populations.
HSCT-TMA is a life-threatening complication, which results from endothelial injury associated with HSCT. The endothelial injury occurs after the lectin pathway of complement is activated.
Narsoplimab, a fully human IgG4 monoclonal antibody, inhibits MASP-2, which is an effector enzyme of the lectin pathway. MASP-2 also activates the coagulation cascade.
On January 19, 2021, the FDA granted a priority review designation to a biologics license application for narsoplimab for the treatment of patients with HSCT-TMA; the FDA is expected to make a decision regarding the approval of narsoplimab by July 17, 2021.2
The analysis utilized data from 4 clinical trials to evaluate the pharmacodynamic effect of narsoplimab on lectin pathway activation. The trials included a pivotal, phase 2 study (NCT02222545), an ongoing phase 2 trial (NCT02682407), and 2 phase 1 healthy volunteer studies. In the phase 2 trials, sparse pharmacokinetics sampling was performed; however, intense sampling was conducted in the healthy volunteers.
Afterward, a population pharmacokinetic/pharmacodynamic model was built based on the subset of single and multiple intravenous (IV) narsoplimab dosing regimens utilized in the 4 clinical trials.
The dataset analyzed included 5031 records including pharmacokinetic observations (n = 3439), infusion events (n = 1567), and C4d inhibition without pharmacokinetics (n = 25).
Of the 110 subjects included in the analysis, 37% were female. Additionally, 23% of patients had atypical hemolytic uremic syndrome, 25% had HSCT-TMA, 15% had IgA nephropathy, and 14% had other diseases. The remaining 23% of participants comprised healthy volunteers.
Regarding pharmacokinetics, minimal accumulation was observed at 2 mg/kg of narsoplimab when given in 6 once-weekly doses. Moderate accumulation was observed at 4 mg/kg.
Narsoplimab appeared to be distributed in the blood and hydrophilic extravascular space because critical parameters for central volume and peripheral volume were consistent with monoclonal antibodies.
Total clearance was concentration-dependent and ranged from 0.1146 to 0.1286 L/h for individual patients. The estimated Michaelis-Menten constant was approximately 5.7 µg/mL.
Additionally, the estimated terminal half-life of narsoplimab was about 198 hours in healthy volunteers who received 6 weekly IV doses at 4 mg/kg.
Covariates that affected the disposition of narsoplimab included albumin level, patient status, body weight, dose, and the presence of anti-drug antibodies (ADA), which were observed in 11% of patients with HSCT-TMA. Patients with ADA positivity had a higher maximum elimination velocity, but only a slightly lower overall exposure.
Taken collectively, the findings support that body weight is a significant covariate of narsoplimab dosing. Linear clearance increased with body weight, so weight-based dosing with narsoplimab is recommended. Conversely, age, race, and sex were not found to significantly affect the disposition of narsoplimab, so dose-adjustment based on any baseline patient characteristic beyond weight is not supported.
Regarding the exposure-response relationship, the EC50 and EC90 values of a direct-link mixed effects Emax model demonstrated that the concentrations of narsoplimab were maintained at levels greater than EC50 throughout the dosing interval.
Additionally, narsoplimab concentrations achieved in patients with HSCT-TMA induced clinically meaningful C4d inhibition and improved clinical response rate. Moreover, the modeling suggested that no residual pharmacodynamic effect was likely to be observed on C4d production 6 weeks after the last dose of narsoplimab.
Also presented during the 2021 EBMT meeting were findings from a pivotal phase 2 trial of narsoplimab (NCT02222545), which demonstrated high response rates and a significant improvement in laboratory markers and organ function irrespective of subgroup in patients with HSCT-TMA.3
Narsoplimab is currently being evaluated in phase 3 trials that are evaluating the agent’s use in other lectin pathway–associated diseases and other endothelial injury syndromes, including IgA nephropathy (NCT03608033) and atypical hemolytic uremic syndrome (NCT03205995).4,5