Edward B. Garon, MD, MS

Edward B. Garon, MD, MS, associate professor of medicine, David Geffen School of Medicine, University of California, Los Angeles, discusses the KEYNOTE-001 study, which examined the 5-year long-term overall survival (OS) in patients with non—small cell lung cancer (NSCLC) treated with pembrolizumab (Keytruda).

The KEYNOTE-001 study was the original study of pembrolizumab, according to Garon. However, it was unlike other first-in-human studies because it enrolled over 1000 patients and led to the initial approvals in melanoma and NSCLC, explained Garon. It also led to approval for an assay to look at PD-L1 expression.

The study showed that the 5-year OS rate was 23.2% in treatment-naïve patients and 15.5% in previously treated patients. The study also showed that the clinical benefit appeared to correlate well with PD-L1 expression, particularly with the best outcomes being amongst patients who had PD-L1 expression in at least half of their tumor cells, according to Garon. The 5-year OS rate in patients with high levels of PD-L1 expression was 29.6% in treatment-naïve patients and 25% in previously treated patients.

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Edward B. Garon, MD, MS, discusses the KEYNOTE-001 study, which examined the 5-year long-term overall survival in patients with non–small cell lung cancer treated with pembrolizumab.

Dr. Garon on the KEYNOTE-001 Study in NSCLC

	at the University of California, Los Angeles

The role of immunotherapy is emerging as an effective, and in some cases, dramatic treatment in the non-small cell lung cancer (NSCLC) landscape. The programmed cell death 1 (PD-1) inhibitor pembrolizumab (Keytruda) is approved for the treatment of previously treated patients with metastatic NSCLC and was originally approved to treat patients with advanced melanoma in 2014.

PD-1 is a negative costimulatory receptor expressed primarily on the surface of activated T cells.

 Binding of PD-1 to one of its ligands, PD-L1, can inhibit T cell cytotoxic responses. Tumors have been hypothesized to utilize this pathway to escape immune surveillance.

Lung cancer is the leading cause of cancer deaths in the United States with NSCLC being responsible for approximately 85% of all cases. According to the National Cancer Institute, in 2015 an estimated 158,040 deaths will result from 221,200 diagnosed lung cancers.

 Despite several available targeted therapies, most patients do not have mutations for which FDA-approved treatments exist.

The large international phase I KEYNOTE-001 trial recently led to accelerated approval for pembrolizumab in NSCLC.

 This study evaluated the efficacy and safety of pembrolizumab in patients with advanced NSCLC. Of the 495 patients reported, 394 had experienced disease progression after undergoing platinum-based chemotherapy. The remaining 101 patients had not been previously treated. Patients received pembrolizumab at a dose of either 2 mg/kg or 10 mg/kg every 3 weeks or 10 mg/kg every 2 weeks.

The anti—PD-L1 antibody clone 22C3 was utilized in an immunohistochemical assay to determine each patient’s PD-L1 expression. Two groups were created, a training group made up of 182 patients and a validation group made up of the remaining 313. During the course of the study, computed tomography or magnetic resonance imaging was performed every 9 weeks and the resultant images were assessed with Response Evaluation Criteria in Solid Tumors (RECIST) version 1.1. In the training group, patients with staining for PD-L1 in at least half of their cells were more likely to clinically respond to pembrolizumab. There were no clear differences between patients with staining in a minority of their cells versus no PD-L1 staining in this training set.

The objective response rate was 19.4%—18.0% in the 394 previously treated patients, and 24.8% in the 101 untreated patients. The best overall response was stable disease in 21.8% of patients. In contrast to current or former smokers who had a response rate of 22.5%, patients who had never smoked cigarettes had a response rate of 10.3%. The response rate for patients with PD-L1 expression in at least half of their cells was 45.2%.

Analysis of median progression-free survival and overall survival in patients with PD-L1 staining in at least half of their cells was 6.1 months for previously treated patients and 12.5 months for previously untreated patients. The median duration of response exceeded a year among those who responded, without clear differences based on PD-L1 expression.

Pembrolizumab showed modest toxicity with fatigue, pruritus, and anorexia being the most common treatment-related adverse events. Serious adverse events were reported in 9.5% of cases, a proportion that is lower than that anticipated with chemotherapy. More specifically, the most common inflammatory or immune-mediated adverse events were hypothyroidism, infusion- related reactions, and pneumonitis.

No significant differences were observed in efficacy or toxicity among patients receiving 10 mg/kg either every 2 or 3 weeks. There were an insufficient number of patients treated with the 2 mg/kg to draw meaningful conclusions.

The KEYNOTE-010 trial compares pembrolizumab to docetaxel in patients with NSCLC whose tumors express PD-L1 and have progressed after systemic platinum-based treatments. This study will be able to answer the relative efficacy of the 2 mg/kg dose and the 10 mg/kg dose.

In the KEYNOTE-001 study, PD-L1 expression was assessed based on tissue collected contemporaneously to treatment instead of archived tissue. As some patients on KEYNOTE-010 underwent new biopsies while others submitted archival biopsies, the role of a new biopsy to assess likelihood of benefit can also be assessed in this phase II/III study.

In addition to conducting the clinical trial, many patients consented for institutional bio-banking protocols. Data from the analysis of those specimens are beginning to emerge, identifying additional factors associated with response to PD-1 inhibition. To date, these evaluations have focused on mutations in the tumor and infiltrating immune cells.

As the malignancies that have been most sensitive to PD-1 inhibition have been those with the highest mutational load, it was hypothesized that tumors with a larger number of mutations would have a greater likelihood of being recognized by the immune system, leading to a correlation between mutational load and sensitivity to pembrolizumab.

Nonsynonymous mutations lead to the creation of neoantigens in tumors, against which activated T cells could mount a robust immune response.

 Whole exome sequencing was performed on 34 patients treated with pembrolizumab at Memorial Sloan Kettering and UCLA. This analysis showed a correlation between mutational load and response to pembrolizumab.

Previous studies in melanoma tumor specimens revealed that responses to PD-1/L1 blockade rely on pretherapy tumor infiltration of activated cytotoxic T cells.

 Initial studies with samples from patients treated with pembrolizumab have shown pre-treatment accumulation of CD8+ T cells, as well as tumor infiltration of CD4+ T cells, corresponding to enhanced durable clinical response (>6 months).

Further analysis of the KEYNOTE-001 trial data revealed that prior tyrosine kinase inhibitor (TKI) therapy in NSCLC EGFR-mutant patients strongly correlated with a lack of response to PD-1 inhibitor therapy. Among the 29 patients treated at UCLA with the EGFR mutation, 2 of 3 patients who were EGFR TKI naïve had a partial response to pembrolizumab. Among the 26 patients who had been previously treated with an EGFR TKI, only 1 had a partial response.

Laboratory studies have shown cell lines treated with EGFR TKIs have lower PD-L1 expression levels.9 The only patient with a prior EGFR TKI who responded to pembrolizumab had an EGFR mutation that is typically not sensitive to available EGFR TKIs. A study of EGFR-mutant TKInaïve NSCLC patients is planned to gain a better understanding of response to PD-1 inhibition in those individuals.

			Keir ME, Butte MJ, Freeman GJ, Sharpe AH. PD-1 and its ligands in tolerance and immunity. 

			Schreiber RD, Old LJ, Smyth MJ. Cancer immunoediting: integrating immunity's roles in cancer suppression and promotion. 

			http://seer.cancer.gov/statfacts/html/lungb.html

			Garon EB, Rizvi NA, Hui R, Leighl N, Balmanoukian AS, Eder JP et al.. Pembrolizumab for the treatment of non-small-cell lung cancer. 

			Alexandrov LB, Nik-zainal S, Wedge DC, et al. Signatures of mutational processes in human cancer. 

			Rizvi NA, Hellmann MD, Snyder A, et al. Cancer immunology. Mutational landscape determines sensitivity to PD-1 blockade in non-small cell lung cancer. 

			Garon EB, Wolf B, Lisberg A, et al. Prior TKI therapy in NSCLC EGFR mutant patients associates with lack of response to anti-PD-1 treatment. Presented at: 2015 World Conference on Lung Cancer; September 6-9; Denver, CO. ID 2172.

			Hu-Lieskovan S, Goldman JW, Han M, et al. High intratumoral T cell infiltration correlated with mutational load and response to pembrolizumab in non-small cell lung cancer. Presented at: 2015 World Conference on Lung Cancer; September 6-9; Denver, CO. ID 2728.

			Tumeh PC, Harview CL, Yearley JH, et al. PD-1 blockade induces responses by inhibiting adaptive immune resistance. 

Articles

The role of immunotherapy is emerging as
an effective, and in some cases, dramatic
treatment in the non-small cell lung
cancer landscape.

Author | Edward B. Garon, MD, MS

Articles

Dr. Garon on the KEYNOTE-001 Study in NSCLC

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