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Opinion|Videos|December 17, 2024

Testing Methodologies and Standards in Metastatic NSCLC

Author(s)Heather A. Wakelee, MD, FASCO, Jacob Sands, MD

Panelists discuss the various tests available for detecting genetic mutations in advanced non–small cell lung cancer (NSCLC), including next-generation sequencing (NGS), reverse transcription polymerase chain reaction (RT-PCR), fluorescence in situ hybridization (FISH), immunohistochemistry (IHC), and Sanger sequencing, and explore whether one method is considered the “gold standard” according to the NCCN guidelines. They also address the appropriate use of circulating tumor DNA (ctDNA) testing, comparing it to tissue testing in this disease state.

Episodes in this series

EP. 1 Patient Case: 67 Year-Old Male With Stage IIIB+ NSCLC Awaiting for Mutation Testing

EP. 1 Patient Case: 67 Year-Old Male With Stage IIIB+ NSCLC Awaiting for Mutation Testing

EP. 2 Next Steps to Stage IIIB NSCLC Patient Case Awaiting Molecular Testing and Usability of Liquid Biopsy

EP. 2 Next Steps to Stage IIIB NSCLC Patient Case Awaiting Molecular Testing and Usability of Liquid Biopsy

EP. 3 Testing Methodologies and Standards in Metastatic NSCLC

EP. 3 Testing Methodologies and Standards in Metastatic NSCLC

EP. 4 Practical Considerations in Metastatic NSCLC: Access and Timing of Testing

EP. 4 Practical Considerations in Metastatic NSCLC: Access and Timing of Testing

EP. 5 Clinical-Decision Making: When to Treat Advanced NSCLC Without Mutational Results

EP. 5 Clinical-Decision Making: When to Treat Advanced NSCLC Without Mutational Results

EP. 6 Initial Approach to Management of RET-Positive NSCLC

EP. 6 Initial Approach to Management of RET-Positive NSCLC

EP. 7 Patient and Therapy Considerations Guiding Initiation of Treatment in RET-Positive NSCLC

EP. 7 Patient and Therapy Considerations Guiding Initiation of Treatment in RET-Positive NSCLC

EP. 8 Considerations for First-Line Targeted Therapies in Metastatic RET-Positive NSCLC

EP. 8 Considerations for First-Line Targeted Therapies in Metastatic RET-Positive NSCLC

EP. 9 Role of Immunotherapy and Chemotherapy in RET-Positive NSCLC

EP. 9 Role of Immunotherapy and Chemotherapy in RET-Positive NSCLC

EP. 10 Managing RET Inhibitor Treatment-Related Adverse Events

EP. 10 Managing RET Inhibitor Treatment-Related Adverse Events

EP. 11 Monitoring Patients With RET-Positive NSCLC

EP. 11 Monitoring Patients With RET-Positive NSCLC

EP. 12 Unmet Needs and Future Perspectives of RET-Positive NSCLC

EP. 12 Unmet Needs and Future Perspectives of RET-Positive NSCLC

Dr Sands to Dr Wakelee: What tests are available for detecting genetic mutations (ie, NGS, RT-PCR, FISH, IHC, Sanger, etc) in advanced NSCLC?
Is one considered the “gold standard” or preferred compared with the others?
NCCN Guidelines Version 10.2024 Non-Small Cell Lung Cancer
Dr. Wakelee: Describe when circulating tumor DNA (ctDNA) testing is appropriate.
How does it compare to tissue testing in this disease state?

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